COLUMN:Ensure the consistency of your retention times with accurate and precise temperature control of standard flow rate applications. The Thermo Scientific™ Dionex™ UltiMate™ TCC-3000SD Standard Thermostatted Column Compartments supports methods at temperatures from 5° to 80°C.
Optional columns switching valves (field upgrade) let you take advantage of application switching or parallel and tandem two-dimensional liquid chromatography (2D-LC) to improve your separations and increase productivity with x2 dual technology.
The UltiMate TCC-3000SD Standard Thermostatted Column Compartments is recommended for use with all Thermo Scientific™ Dionex™ UltiMate™ 3000 Standard (SD) LC systems.
Features
- Temperature range of 5° to 80°C ±0.5°C and stability of ±0.1°C simplifies method optimization.
- Precise temperature control under varying ambient conditions ensures retention time precision.
- Fast heat-up/cool down times minimize delays when changing between methods.
- Eluent preheaters deliver sharper peaks and better resolution.
PUMP:
Increase your binary method flexibility. The Thermo Scientific Dionex UltiMate HPG-3400SD Standard Binary Pump allows four different mobile phases, two at a time. Thermo Scientific SmartFlow technology delivers pulse-free flows without a damper to ensure precise retention times. With support for pressures up to 62MPa (9,000 psi/620 bar) at flow rates up to 10mL/min., UltiMate 3000 SD pumps excel in standard LC applications and offer full compatibility with fast UHPLC separations, including short sub-2 µm particle columns for faster analysis and lower solvent consumption.
This pump is recommended for use within the UltiMate 3000 Binary Standard system. The HPG-3400SD pump is ideal for routine analyses with an added measure of flexibility using conventional LC and high-throughput LC in the pharmaceutical, food and beverage and environmental industries.
UltiMate 3000 Standard SD and Rapid Separation (RS) pumps and system configurations provide similar gradient delay and extra-column volumes. This simplifies the method transfer between method development labs commonly using UHPLC pumps and QC and production labs with their standard LC pump setups.
Features:
- Serial dual-piston operating principle, high-pressure gradient proportioning
- Wide operating flow rate range from 100µL to 10mL/min. with high flow rate accuracy
- Support of operating pressures of up to 62 MPa (9,000 psi/620 bar)
- Thermo Scientific SmartFlow technology to automatically compensate for compressibility; no manual solvent setting needed
- Large selection of mixing volumes to perfectly balance gradient delay volume with mixing efficiency
- Active rear-seal wash and floating pistons to maximize seal lifetime and pump uptime
Autosampler:
Enjoy wide application flexibility in a cost-effective, dependable autosampler. The Thermo Scientific™ Dionex™ UltiMate™ Standard Well Plate Autosampler is highly versatile in sample format and injection volume. It can rapidly, accurately and precisely inject from 1 to 100μL for conventional LC and up to 62 MPa (9000 psi) for UHPLC applications. The in-line split-loop (flow-through) injection design puts the needle and sample loop directly in the high-pressure fluid path. Combined with an external needle wash option, carryover is essentially eliminated.
Flexible Formats for Low-Flow Applications
The sample rack handles up to three well plates (96 or 384 wells—sealed or unsealed) or sample vial trays in any combination. Run your samples in the same format used for preparation. You can even exchange sample plates during a run. An optional cooler preserves thermolabile samples by chilling to 4°C. The UltiMate 3000 RSLCnano Well Plate Autosampler (WPS-3000TPLRS) is the ideal solution for chromatographers looking to work with the highest injection precision at nano, capillary and micro flow rates in UHPLC applications.
Maximize Throughput
- Three-well plate capacity holds up to 1152 samples
- Large injection range from 10nL to 125μL offers application flexibility
- Stable injection valve pressure extends up to 90MPa
- Thermostatted sample compartment keeps temperature constant from 4 to 45°C or 22°C below ambient
Ensure Accurate Small Sample Performance
- Covered and thermostatted sample tray to protect thermo- and UV-labile samples
- Dual-needle injection principle to minimize sample loss
- Injection precision <0.25% RSD at 5μL (typically <0.15% RSD)
- Low carryover (typically <0.01%)
- Injection Mechanism
Using the dual-needle principle, the vial septum of the well seal is pierced by the stainless steel outer needle. The sampler guides the fused silica capillary sampling needle through the piercing needle down to the bottom of the sample carrier. Sample needle height is easily programmed to match sample vial or well depth. Injections of minute sample volumes with virtually zero sample loss—1μL injection per 1μL sample—are performed using the μL-pickup routine. This routine is of particular importance when injecting protein samples that cannot be diluted without the risk of protein loss or alteration.
