Condition: Refurbished
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This Applied Biosystems QuantStudio 12K Flex Real-Time PCR System (REF 4470661, S/N 285880907, manufactured June 2015) is fitted with a 96-well fast block and runs firmware v1.4.0. The unit has passed all self-diagnostic tests and was professionally serviced by Thermo Fisher Scientific on 13 January 2025, with next service due January 2026.

Included in the sale:
• Applied Biosystems QuantStudio 12K Flex Real-Time PCR System (REF 4470661)
• 96-Well Fast Block with plate holder
• Power cable

The images provided are of the actual item you will receive. Please review them carefully for the cosmetic condition and what is included in the sale.

The Applied Biosystems QuantStudio 12K Flex Real-Time PCR System is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems real-time technology in a single instrument. It is designed to take researchers from targeted discovery through confirmation and screening, all on a single platform, scaling from a single reaction to 12,000 data points per run.

Key Features:
- Five interchangeable block formats (96-well standard, 96-well Fast, 384-well, TaqMan Array Micro Fluidic Cards, OpenArray plates) changeable from the front of the instrument in under 1 minute without secondary tools
- Enhanced OptiFlex System: White LED excitation source with 6 excitation filters (450–670 nm) and 6 emission filters (500–720 nm), supporting 21 colour combinations
- Up to 9 logs of linear dynamic range (up to 7 logs with OpenArray); detects as little as 1.5-fold changes in target quantities in singleplex
- Capable of running up to four 3,072-reaction OpenArray plates simultaneously in approximately four hours
- Combined with the AccuFill System: up to 110,000 data points in an 8-hour day with as little as 20 minutes hands-on time per run
- Intuitive touchscreen interface with instrument control via touch screen, networked computer, or attached computer
- Remote monitoring of up to 10 instruments simultaneously; stores over 100 runs on-instrument
- Compatible with the full range of TaqMan assays for gene expression, microRNA, SNP genotyping, copy number variation, protein thermal shift, high resolution melt, and pathogen detection
- Seamless switch between qPCR and digital PCR mode using OpenArray block and DigitalSuite Software
- LIMS support and optional 21 CFR Part 11 compliance module (security, audit, and e-signature)
- Automation compatible with plate-loading arm and barcode reader
- Noise output: <60 dB at idle

Specifications:
Catalogue Number: 4470661
Supported Block Formats: 96-well standard (10–100 µL), 96-well Fast (10–30 µL), 384-well (5–20 µL), TaqMan Array Card (~1 µL), OpenArray Plate (~33 nL)
Excitation Source: Enhanced OptiFlex System — White LED
Filter Channels: 6 excitation / 6 emission (21 colour combinations)
Excitation Range: 450–670 nm
Emission Range: 500–720 nm
Dynamic Range: Up to 9 logs (96-well/384-well); up to 7 logs (OpenArray)
Sensitivity: Down to 1 copy
Resolution: Detects as little as 1.5-fold change in singleplex
Run Time (Fast 96-well): ~30 minutes
Run Time (384-well): ~35 minutes
Run Time (OpenArray, gene expression): ~2 hours
Run Time (OpenArray, genotyping): ~4 hours
Max Throughput: Up to 110,000 data points per 8-hour day (with AccuFill System)
Instrument Control: Touchscreen, networked computer, or attached computer
Power Rating: 100–240 VAC, 50/60 Hz
Dimensions (W x D x H): 20 x 26.3 x 29.1 in (51 x 67 x 74 cm)
Weight: 69 kg (152 lbs)
Noise: <60 dB at idle
Compliance: Optional 21 CFR Part 11 module; EN61326 Class B Group 1
Connectivity: Ethernet, USB (x4), RS232
For Research Use Only. Not for use in diagnostic procedures.

QuantStudio 12K Flex Real-Time PCR System — Maintenance and Administration User Guide (v1.5): Download PDF

QuantStudio 12K Flex Real-Time PCR System — Multi-Well Plates and Array Card Quick Reference Guide: Download PDF

QuantStudio 12K Flex Real-Time PCR System — OpenArray Plate Quick Reference Guide: Download PDF

QuantStudio 12K Flex Real-Time PCR System — Site Preparation Guide (Pub. No. 4470654): Download PDF

QuantStudio 12K Flex Real-Time PCR System — Multi-Well Plates Quick Reference (cms_101435): Download PDF

QuantStudio 12K Flex Real-Time PCR System — Product brochure (Stanford HTBC): Download PDF