illumina iSeq 100 Next Generation Sequencing System 20021532 MFD 2022 SBS

Condition: Used — In excellent condition, tested and working. Sourced from a lab closure. Manufactured 2022.

This is the Illumina iSeq 100 Next Generation Sequencing System (part number 20021532), combining Illumina's Sequencing by Synthesis (SBS) chemistry with CMOS sensor technology in a compact benchtop form factor. The unit has been confirmed functional and is ready for laboratory use.

Included in the sale:
• Power adapter
• Ethernet cable
• USB cable
• Software

The images provided are of the actual item you will receive. Please review them carefully for the cosmetic condition and what is included in the sale.

Harness the power of Illumina next-generation sequencing (NGS) technology in an easy-to-use, cost-effective ecosystem designed for the benchtop. The Illumina iSeq 100 delivers highly accurate data, fast run times, and a maximum output of 1.2 Gb in a simple, compact form factor.

The iSeq 100 is a compact and affordable benchtop sequencer designed for smaller labs or targeted sequencing applications. It fills a specific niche in the NGS market by providing fast, accessible, and high-quality sequencing data for projects that do not require the throughput of larger production-scale instruments. The iSeq 100 maintains the accuracy and reliability associated with Illumina's Sequencing by Synthesis (SBS) chemistry.

Key Innovations

CMOS Sensor Technology: The iSeq 100 utilises a complementary metal-oxide semiconductor (CMOS) image sensor chip, similar to those found in smartphone cameras. Paired with a specialised one-channel SBS chemistry, this replaces conventional laser optics and camera systems, simplifying the instrument, reducing cost, and delivering a smaller footprint — all while maintaining data accuracy.

Simplified Workflow: Prefilled reagent cartridges and an intuitive software interface minimise hands-on time, making NGS accessible to labs without dedicated sequencing specialists.

Key Specifications

Sequencing Chemistry: Sequencing by Synthesis (SBS), one-channel
Sensor Technology: CMOS image sensor chip
Flow Cell: Single-lane flow cell
Maximum Data Output: 1.2 Gb per run
Run Time: Up to 19 hours
Read Length: Up to 2 × 150 bp (paired-end)
Read Type: Single-read and paired-end
Instrument Dimensions: 26 cm (W) × 36 cm (D) × 26 cm (H)
Instrument Weight: Approximately 15 kg
Part Number: 20021532

Intended Applications

Small Whole-Genome Sequencing: Bacteria, viruses, and plasmids using Illumina DNA Prep or Nextera XT Library Prep Kits.
Targeted Gene Sequencing: Gene panels including TruSight One or AmpliSeq Custom Panels.
Gene Expression Profiling: RNA expression measurement with Illumina Stranded mRNA Prep.
16S Metagenomics: Microbial community characterisation using the Illumina 16S Metagenomic Sequencing Protocol.

The iSeq 100 is particularly well suited to labs prioritising ease of use, affordability, and fast turnaround times for lower-throughput sequencing projects. Its streamlined design makes professional-grade NGS accessible without the complexity or specialist expertise required by larger sequencing platforms.

Illumina iSeq 100 System Guide — official instrument manual (PDF): Download PDF