illumina iSeq 100 Next Generation Sequencing System 20021532 MFD 2022 SBS
Model: iSeq 100
Condition: Used
In stock: 0
SKU: 2885 2A03
- Misc Test Equipment
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Condition: Used
In excellent condition, tested and working. Sourced from a lab closure. Manufactured 2022. Express worlwide shipping.
Supplied with a power adapter, ethernet and USB with software.
Harness the power of Illumina next-generation sequencing (NGS) technology in an easy-to-use, inexpensive ecosystem right in your lab
Highly accurate data, fast run times, and a maximum output of 1.2 Gb in a simple, compact benchtop sequencing system
The Illumina iSeq 100 is a compact and affordable benchtop sequencer designed for smaller labs or targeted sequencing applications. It fills a specific niche in the next-generation sequencing (NGS) market by providing fast, accessible, and high-quality data for projects that don't require the throughput of larger production-scale sequencers. The iSeq 100 maintains the accuracy and reliability associated with Illumina's sequencing by synthesis (SBS) chemistry.
Key Innovations
CMOS Sensor Technology: The iSeq 100 sets itself apart by utilizing a complementary metal-oxide semiconductor (CMOS) image sensor chip, similar to those found in smartphone cameras. This chip is paired with a specialized one-channel SBS chemistry designed for optimal signal detection. By replacing conventional laser optics and camera systems, the CMOS sensor design simplifies the instrument significantly, reduces instrument cost and results in a smaller footprint, while still delivering accurate data.
Simplified Workflow: The iSeq 100 boasts a streamlined setup and operation process. Prefilled reagent cartridges and an intuitive software interface minimize hands-on time, making NGS accessible to labs without dedicated sequencing specialists.
Features and Technologies
Sequencing by Synthesis (SBS): The iSeq 100 employs Illumina's proven SBS chemistry, using fluorescently labeled nucleotides to generate highly accurate sequencing data.
Single-Lane Flow Cell: The iSeq 100's flow cell has a single sequencing lane, optimized for smaller output runs.
Data Output: Capable of producing up to 1.2 Gb of data per run.
Run Time: Sequencing runs can be completed in 19 hours or less.
Read Length: Supports up to 2 x 150 bp (paired-end) reads.
Intended Uses and Specific Applications
The iSeq 100's capabilities make it ideal for various research applications, including:
Small Whole-Genome Sequencing: Sequencing of smaller genomes like bacteria, viruses, and plasmids. Suitable library prep kits include Illumina DNA Prep Library Prep Kit or Nextera XT DNA Library Prep Kit.
Targeted Gene Sequencing: Analysis of specific genes or regions of interest (panels). Suitable kits include TruSight One Panels or AmpliSeq Custom Panels.
Gene Expression Profiling: Measuring RNA expression using kits like Illumina Stranded mRNA Prep.
16S Metagenomics: Characterization of microbial communities. Suitable library prep includes Illumina DNA Prep Library Prep Kit or the Illumina 16S Metagenomic Sequencing Protocol.
Comparison with Competing Instruments
Illumina MiniSeq: The MiniSeq offers higher throughput (up to 7.5 Gb per run). It's a better choice for projects requiring larger sequencing output, but it comes with increased instrument cost and complexity.
Complete Genomics DNBSEQ-E25: This platform offer higher max output and similar speeds, but it cannot offer the shorter sequencing read lengths available to the iSeq 100 and may come at a higher upfront cost.
Special Considerations
The iSeq 100 is a compelling option when these factors are important:
Ease of Use: Ideal for labs that want reliable NGS data without the complexity or in-house expertise requirements of larger sequencing platforms.
Affordability: Offers a lower initial investment compared to larger Illumina sequencers or comparable competitors.
Fast Turnaround Time: Delivers results quickly when time is a critical factor.
Sequencing by synthesis chemistry
The iSeq 100 System harnesses proven Illumina sequencing by synthesis (SBS) chemistry to deliver highly accurate data and robust performance for a broad range of applications. SBS chemistry uses reversible-terminator fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands, reading billions of sequences in parallel.
One-channel SBS chemistryThe iSeq 100 System combines Illumina SBS chemistry with complementary metal-oxide semiconductor (CMOS) technology to deliver one-channel SBS chemistry. One-channel SBS chemistry on a CMOS chip helps to reduce instrument cost and allows for a smaller benchtop footprint, all while delivering accurate data.
Product Overview
Condition: Used
In excellent condition, tested and working. Sourced from a lab closure. Manufactured 2022. Express worlwide shipping.
Supplied with a power adapter, ethernet and USB with software.
Manufacturer's Description
Harness the power of Illumina next-generation sequencing (NGS) technology in an easy-to-use, inexpensive ecosystem right in your lab
Highly accurate data, fast run times, and a maximum output of 1.2 Gb in a simple, compact benchtop sequencing system
The Illumina iSeq 100 is a compact and affordable benchtop sequencer designed for smaller labs or targeted sequencing applications. It fills a specific niche in the next-generation sequencing (NGS) market by providing fast, accessible, and high-quality data for projects that don't require the throughput of larger production-scale sequencers. The iSeq 100 maintains the accuracy and reliability associated with Illumina's sequencing by synthesis (SBS) chemistry.
Key Innovations
CMOS Sensor Technology: The iSeq 100 sets itself apart by utilizing a complementary metal-oxide semiconductor (CMOS) image sensor chip, similar to those found in smartphone cameras. This chip is paired with a specialized one-channel SBS chemistry designed for optimal signal detection. By replacing conventional laser optics and camera systems, the CMOS sensor design simplifies the instrument significantly, reduces instrument cost and results in a smaller footprint, while still delivering accurate data.
Simplified Workflow: The iSeq 100 boasts a streamlined setup and operation process. Prefilled reagent cartridges and an intuitive software interface minimize hands-on time, making NGS accessible to labs without dedicated sequencing specialists.
Features and Technologies
Sequencing by Synthesis (SBS): The iSeq 100 employs Illumina's proven SBS chemistry, using fluorescently labeled nucleotides to generate highly accurate sequencing data.
Single-Lane Flow Cell: The iSeq 100's flow cell has a single sequencing lane, optimized for smaller output runs.
Data Output: Capable of producing up to 1.2 Gb of data per run.
Run Time: Sequencing runs can be completed in 19 hours or less.
Read Length: Supports up to 2 x 150 bp (paired-end) reads.
Intended Uses and Specific Applications
The iSeq 100's capabilities make it ideal for various research applications, including:
Small Whole-Genome Sequencing: Sequencing of smaller genomes like bacteria, viruses, and plasmids. Suitable library prep kits include Illumina DNA Prep Library Prep Kit or Nextera XT DNA Library Prep Kit.
Targeted Gene Sequencing: Analysis of specific genes or regions of interest (panels). Suitable kits include TruSight One Panels or AmpliSeq Custom Panels.
Gene Expression Profiling: Measuring RNA expression using kits like Illumina Stranded mRNA Prep.
16S Metagenomics: Characterization of microbial communities. Suitable library prep includes Illumina DNA Prep Library Prep Kit or the Illumina 16S Metagenomic Sequencing Protocol.
Comparison with Competing Instruments
Illumina MiniSeq: The MiniSeq offers higher throughput (up to 7.5 Gb per run). It's a better choice for projects requiring larger sequencing output, but it comes with increased instrument cost and complexity.
Complete Genomics DNBSEQ-E25: This platform offer higher max output and similar speeds, but it cannot offer the shorter sequencing read lengths available to the iSeq 100 and may come at a higher upfront cost.
Special Considerations
The iSeq 100 is a compelling option when these factors are important:
Ease of Use: Ideal for labs that want reliable NGS data without the complexity or in-house expertise requirements of larger sequencing platforms.
Affordability: Offers a lower initial investment compared to larger Illumina sequencers or comparable competitors.
Fast Turnaround Time: Delivers results quickly when time is a critical factor.
Sequencing by synthesis chemistry
The iSeq 100 System harnesses proven Illumina sequencing by synthesis (SBS) chemistry to deliver highly accurate data and robust performance for a broad range of applications. SBS chemistry uses reversible-terminator fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands, reading billions of sequences in parallel.
One-channel SBS chemistryThe iSeq 100 System combines Illumina SBS chemistry with complementary metal-oxide semiconductor (CMOS) technology to deliver one-channel SBS chemistry. One-channel SBS chemistry on a CMOS chip helps to reduce instrument cost and allows for a smaller benchtop footprint, all while delivering accurate data.
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