Condition: Used

A used Applied Biosystems StepOnePlus Real-Time PCR System (cat. no. 4376592), manufactured in 2011. Cycle count is very low, indicating minimal usage. The system has undergone comprehensive testing including cycle performance and optical verification, and has passed all assessments.

Included with this system:
• Applied Biosystems StepOnePlus Real-Time PCR System (4376592)
• Power cable

The Applied Biosystems StepOnePlus Real-Time PCR System is a 96-well, 4-colour quantitative real-time PCR instrument designed for both first-time and experienced users. Utilising a robust LED-based optical system and VeriFlex Block Technology, it delivers precise, reproducible qPCR results for a broad range of genomic research applications. The system can be operated as a standalone (PC-free) instrument, connected via USB, or integrated into a laboratory network via LAN. It is factory-calibrated for optical and thermal accuracy and ships ready to use.

Key Features:
- VeriFlex Block Technology: Six independently controlled temperature zones for precise and uniform thermal cycling
- LED-Based 4-Colour Optical System: Long-life blue LED excitation source and photodiode detection across four emission filters
- 6.5-inch VGA LCD Touchscreen: Intuitive on-instrument interface for standalone operation
- Fast and Standard Cycling Modes: Fast mode completes a 40-cycle run in under 40 minutes; Standard mode in under 2 hours
- Flexible Data Management: PC-free operation with USB data export; network connectivity for remote monitoring and email notifications
- StepOne Software: Supports Comparative Ct, Standard Curve, Relative Standard Curve, Genotyping/Presence-Absence, Melt Curve, and High-Resolution Melt (HRM) analysis methods
- Factory Calibrated: Calibrated for optical and thermal accuracy prior to shipment

Instrument Specifications:
Format: 96-well plate, 8-tube strips, 0.1 mL tubes
Capacity: 96 x 0.1 mL tubes, 12 x 8-tube strips, 1 x 96-well plate
Reaction Volume Range: 10–30 µL (Standard curve experiments: 40 µL in Standard mode validated)
Temperature Range: 4°C to 100°C
Temperature Accuracy: ±0.25°C (35°C to 95°C)
Temperature Uniformity: ±0.50°C (35°C to 95°C)
Sample Ramp Rate: Fast mode ±2.2°C/s; Standard mode ±1.6°C/s
Peak Block Ramp Rate: 4.6°C/s
Sensitivity: 1 copy
Linear Dynamic Range: Greater than 9 log units
Optical System: Blue LED excitation source; photodiode; 4 emission filters
Calibrated Dyes: FAM, VIC, JOE, NED, ROX, SYBR Green I, TAMRA
Passive Reference Dye: ROX (no ROX, pre-mixed, or separate tube options)
Thermal Cycling System: Peltier-based
Display: 6.5-inch VGA LCD (640 x 480), 32K colours
Data Export Formats: JPEG, Excel, PowerPoint, RDML
Dimensions (W x D x H): 24.6 cm x 42.7 cm x 51.2 cm (9.7 in x 16.8 in x 20.2 in)
Weight: 24 kg (53 lb)
Connectivity: USB, Ethernet (LAN)

Supported Applications:
Gene Expression Analysis (Comparative Ct and Standard Curve)
SNP Genotyping
Pathogen Detection
Viral Load Analysis
microRNA Expression
Protein Expression Analysis
Translocation Analysis
High-Resolution Melt (HRM) Analysis

For Research Use Only. Not for use in diagnostic procedures.

Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems — Official Product Brochure (Thermo Fisher): Download PDF

Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems — Getting Started Guide for Standard Curve Experiments: Download PDF

Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems — Maintenance and Networking User Guide: Download PDF

Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems — Comprehensive User Guide (4376782): Download PDF

Applied Biosystems StepOne and StepOnePlus — RNA-to-Ct User Bulletin: Download PDF

StepOne and StepOnePlus Real-Time PCR Systems — Product Brochure (gene-quantification.de mirror): Download PDF

Shipping & Returns Policy

Shipping Policy

• Processing Time: Orders are typically processed and dispatched within 1 business day.
• Shipping Methods: We offer Expedited Shipping to all destinations using UPS/DHL/Fedex/Royal Mail and other reputable carriers.
• Shipping Times: Mainland UK: 1-2 Business Days / Mainland Europe and USA: 2-3 Business Days / Rest of the World 5-7 Business Days.
• Shipping Costs: These are calculated at checkout based on the shipping method, destination and package dimensions.
• CUSTOMS AND TAXES: It is YOUR responsibility to liaise with customs in your country and ensure your package is cleared and any taxes are paid.
• Shipping Destinations: We ship worldwide.
• Tracking: Once your order is shipped, we will provide you with a tracking number via email.
• Insurance: All shipments are insured. In case of damage, please contact us via email within 48 hours of receiving the product with photographic evidence, please also ensure a report is logged with the delivery driver.

Returns Policy

• Return Period: In the event of an item being received which is 'not as specified', you can return your purchase within 14 days of delivery.
• Condition of Items: Items must be returned unused and in their original packaging.
• Return Method: All returns must be notified in writing to hello@ttinstruments.com, the email should accompany the order number, date and reason for return and must be within 14 days of delivery.
• Restocking Fees: A restocking fee of up-to 20% is applicable for items returned for any reason other than 'not as specified'.
• Refund Method: Refunds are processed to the original method of payment within 5 business days of receiving the return.
• Exchanges: We do not offer exchanges. It will either be a replacement or a refund.
• Return Shipping Costs: Return shipping costs will be borne by the customer unless the item is 'not as specified', in this case we will arrange for the return by way of a prepaid shipping label.