











Applied Biosystems QuantStudio 5 Real-Time PCR Instrument 384-Well Block A28135
Model: QuantStudio 5
Condition: Used
In stock: 0
SKU: 3325 1C02
- Biotech
- Laboratory
- PCR Instrument
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Condition: Used
Applied Biosystems QuantStudio 5 Real-Time PCR Instrument with 384-Well Block, manufactured in 2020. The instrument is in excellent condition, passes self-test, and is fully functional for running protocols in guest mode. Note: a 4-digit numerical admin PIN is required to reset to factory settings or access certain maintenance functions — this PIN is not available. Please contact TT Instruments for a shipping quote prior to ordering.
What's included:
• Applied Biosystems QuantStudio 5 Real-Time PCR Instrument with 384-Well Block (A28135)
The Applied Biosystems QuantStudio 5 Real-Time PCR System (catalogue number A28135) is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The 384-well configuration is the highest-throughput block option available for the QuantStudio 5 platform, supporting up to 5-target multiplexing via OptiFlex technology with 5 coupled excitation and emission filter channels driven by a bright white LED.
The system features an interactive touchscreen for on-instrument experiment setup and run management, and integrates with QuantStudio Design and Analysis Desktop Software or the cloud-based Thermo Fisher Connect platform for remote data access and analysis. Built-in software features support compliance with 21 CFR Part 11 guidelines, including user account management, locked workflow, and e-signature capabilities.
Key Features:
• OptiFlex technology with 5 coupled filter channels and white LED excitation for accurate well-to-well and instrument-to-instrument reproducibility
• Detects changes in target quantity as small as 1.5-fold in single-plex reactions
• 10 logs of linear dynamic range
• Run times under 35 minutes with fast master mix
• Compatible with probe-based assays and intercalating dyes (FAM/SYBR Green, VIC/JOE/HEX/TET, TAMRA/NED/ABY, ROX/Texas Red, LIZ/Cy5/Mustang Purple)
• Applications include gene expression, genetic variation, genotyping, gene regulation, and library quantification
Technical Specifications (384-Well Block):
Block Format: 384-well
Sample Volume Range: 5–20 µL
Maximum Ramp Rate: 6.0°C/second
Average Sample Ramp Rate: 2.92°C/second
Temperature Uniformity: ±0.4°C
Temperature Accuracy: ±0.25°C
Temperature Range: 4–100°C
Optical Detection: 5 coupled filters
Excitation Range: 450–650 nm
Emission/Detection Range: 500–700 nm
Multiplexing: Up to 5 targets
Dynamic Range: 10 logs
Detection Sensitivity: 1.5-fold differences in target quantities; down to 1 copy
Run Time: <35 minutes (with fast master mix)
Power Supply: 100–240 VAC, 50/60 Hz
Max Power Consumption: 960 W
Dimensions (W × D × H): 27 × 50 × 40 cm (10.6 × 19.7 × 15.7 in)
Weight: 25.8 kg (56.9 lb)
For Research Use Only. Not for use in diagnostic procedures.
QuantStudio 3 and 5 Real-Time PCR Systems — Official Specification Sheet (PDF): Download PDF
QuantStudio 3 and 5 Real-Time PCR Systems — Installation, Use, and Maintenance User Guide (PDF): Download PDF
QuantStudio Design and Analysis Desktop Software User Guide MAN0010408 (PDF): Download PDF
QuantStudio 5 Real-Time PCR System — Product Brochure (PDF): Download PDF
QuantStudio 5 Real-Time PCR Instrument Site Preparation Guide MAN0016701 (PDF): Download PDF
Product Overview
Condition: Used
Applied Biosystems QuantStudio 5 Real-Time PCR Instrument with 384-Well Block, manufactured in 2020. The instrument is in excellent condition, passes self-test, and is fully functional for running protocols in guest mode. Note: a 4-digit numerical admin PIN is required to reset to factory settings or access certain maintenance functions — this PIN is not available. Please contact TT Instruments for a shipping quote prior to ordering.
What's included:
• Applied Biosystems QuantStudio 5 Real-Time PCR Instrument with 384-Well Block (A28135)
Manufacturer's Description
The Applied Biosystems QuantStudio 5 Real-Time PCR System (catalogue number A28135) is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The 384-well configuration is the highest-throughput block option available for the QuantStudio 5 platform, supporting up to 5-target multiplexing via OptiFlex technology with 5 coupled excitation and emission filter channels driven by a bright white LED.
The system features an interactive touchscreen for on-instrument experiment setup and run management, and integrates with QuantStudio Design and Analysis Desktop Software or the cloud-based Thermo Fisher Connect platform for remote data access and analysis. Built-in software features support compliance with 21 CFR Part 11 guidelines, including user account management, locked workflow, and e-signature capabilities.
Key Features:
• OptiFlex technology with 5 coupled filter channels and white LED excitation for accurate well-to-well and instrument-to-instrument reproducibility
• Detects changes in target quantity as small as 1.5-fold in single-plex reactions
• 10 logs of linear dynamic range
• Run times under 35 minutes with fast master mix
• Compatible with probe-based assays and intercalating dyes (FAM/SYBR Green, VIC/JOE/HEX/TET, TAMRA/NED/ABY, ROX/Texas Red, LIZ/Cy5/Mustang Purple)
• Applications include gene expression, genetic variation, genotyping, gene regulation, and library quantification
Technical Specifications (384-Well Block):
Block Format: 384-well
Sample Volume Range: 5–20 µL
Maximum Ramp Rate: 6.0°C/second
Average Sample Ramp Rate: 2.92°C/second
Temperature Uniformity: ±0.4°C
Temperature Accuracy: ±0.25°C
Temperature Range: 4–100°C
Optical Detection: 5 coupled filters
Excitation Range: 450–650 nm
Emission/Detection Range: 500–700 nm
Multiplexing: Up to 5 targets
Dynamic Range: 10 logs
Detection Sensitivity: 1.5-fold differences in target quantities; down to 1 copy
Run Time: <35 minutes (with fast master mix)
Power Supply: 100–240 VAC, 50/60 Hz
Max Power Consumption: 960 W
Dimensions (W × D × H): 27 × 50 × 40 cm (10.6 × 19.7 × 15.7 in)
Weight: 25.8 kg (56.9 lb)
For Research Use Only. Not for use in diagnostic procedures.
Resources
QuantStudio 3 and 5 Real-Time PCR Systems — Official Specification Sheet (PDF): Download PDF
QuantStudio 3 and 5 Real-Time PCR Systems — Installation, Use, and Maintenance User Guide (PDF): Download PDF
QuantStudio Design and Analysis Desktop Software User Guide MAN0010408 (PDF): Download PDF
QuantStudio 5 Real-Time PCR System — Product Brochure (PDF): Download PDF
QuantStudio 5 Real-Time PCR Instrument Site Preparation Guide MAN0016701 (PDF): Download PDF
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