Condition: Used

This Applied Biosystems QuantStudio 5 Dx Real-Time PCR instrument was manufactured in 2022 and is in excellent condition, passing the on-board self-test. The system is currently operating in SAE (Security, Auditing and E-signature) mode — a PIN-protected access control mode required for IVD regulatory compliance. Running experiments requires a valid user or admin PIN to log in; TT Instruments does not hold the current PIN credentials for this instrument. Prospective buyers are advised to confirm suitability before ordering.

What is typically included with this product (per listing description):
• QuantStudio 5 Dx instrument (96-well, 0.2 mL block)

The Applied Biosystems QuantStudio 5 Dx Real-Time PCR System is an in vitro diagnostic (IVD) quantitative PCR system designed for clinical and hospital laboratories and assay developers. It supports both Test Development (DEV) and In Vitro Diagnostic (IVD) operating modes within a single software interface, helping to minimise the risk of operating in the wrong mode. The system is both US-IVD and CE-IVD, IVDR compliant.

Key features include OptiFlex technology, which decouples excitation and emission filters across 6 channels (with up to 21 filter combinations) for optimised fluorescent dye performance and reliable multiplexing. VeriFlex thermal blocks allow precise independent temperature control across six zones of the 96-well sample block, delivering consistent and reproducible PCR amplification. The instrument detects differences in target quantity as small as 1.5-fold in singleplex reactions, with 10 logarithmic units of linear dynamic range.

The QuantStudio 5 Dx Dx software includes Security, Auditing and E-signature (SAE) features to support compliance with 21 CFR Part 11 and EU IVD regulations. The IVD test menu restricts operation to authorised IVD tests only, reducing the risk of accidental misuse. Subsequent IVD dye calibrations are user-enabled to minimise ongoing operating costs.

Technical Specifications (96-well, 0.2 mL block):
Block Format: 96-well plate, 0.2 mL
Reaction Volume Range: 10–100 µL
Maximum Heating Rate: 6.5°C/sec
Cooling Rate: 3.66°C/sec
Temperature Range (Block): 4–99.9°C
Heated Cover Temperature Range: 50–110°C
Temperature Zones: 6 independent VeriFlex zones
Optical Channels: 6 decoupled filters, up to 21 combinations
Excitation/Emission Range: 450–680 nm / 500–730 nm
Light Source: Bright white LED (median lifetime >5 years)
Multiplex Capability: Up to 6 targets
Run Time: Less than 30 minutes (typical)
Dye Compatibility: FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ, Cy5.5
Sensitivity: 1.5-fold change detection in singleplex reactions
Dynamic Range: 10 logarithmic units
Dimensions: 27 W x 50 D x 40 H cm
On-Board Memory: 10 GB
Connectivity: USB, Ethernet
Regulatory Status: CE-IVD (IVDR), US-IVD
Software Modes: Test Development (DEV) and In Vitro Diagnostic (IVD)
Compliance: 21 CFR Part 11, SAE (Security, Auditing, E-signature)

Typical Applications: Pathogen detection (viruses, bacteria, fungi), gene expression analysis, SNP genotyping, mutation analysis, companion diagnostic development, clinical molecular diagnostics.

QuantStudio 5 Dx Real-Time PCR System — Official Specification Sheet (Thermo Fisher): Download PDF

QuantStudio 3 and 5 Real-Time PCR Systems — Specification Sheet (Fisher Scientific): Download PDF

QuantStudio 3 and 5 Real-Time PCR Systems — Installation, Use and Maintenance Guide (User Manual): Download PDF

QuantStudio 5 Dx US-IVD Sole Source Reference Document (Thermo Fisher Americas): Download PDF

QuantStudio 5 Dx Assay Developer Reference Document (Thermo Fisher Americas): Download PDF

Shipping & Returns Policy

Shipping Policy

• Processing Time: Orders are typically processed and dispatched within 1 business day.
• Shipping Methods: We offer Expedited Shipping to all destinations using UPS/DHL/Fedex/Royal Mail and other reputable carriers.
• Shipping Times: Mainland UK: 1-2 Business Days / Mainland Europe and USA: 2-3 Business Days / Rest of the World 5-7 Business Days.
• Shipping Costs: These are calculated at checkout based on the shipping method, destination and package dimensions.
• CUSTOMS AND TAXES: It is YOUR responsibility to liaise with customs in your country and ensure your package is cleared and any taxes are paid.
• Shipping Destinations: We ship worldwide.
• Tracking: Once your order is shipped, we will provide you with a tracking number via email.
• Insurance: All shipments are insured. In case of damage, please contact us via email within 48 hours of receiving the product with photographic evidence, please also ensure a report is logged with the delivery driver.

Returns Policy

• Return Period: In the event of an item being received which is 'not as specified', you can return your purchase within 14 days of delivery.
• Condition of Items: Items must be returned unused and in their original packaging.
• Return Method: All returns must be notified in writing to hello@ttinstruments.com, the email should accompany the order number, date and reason for return and must be within 14 days of delivery.
• Restocking Fees: A restocking fee of up-to 20% is applicable for items returned for any reason other than 'not as specified'.
• Refund Method: Refunds are processed to the original method of payment within 5 business days of receiving the return.
• Exchanges: We do not offer exchanges. It will either be a replacement or a refund.
• Return Shipping Costs: Return shipping costs will be borne by the customer unless the item is 'not as specified', in this case we will arrange for the return by way of a prepaid shipping label.