Condition: Used Thermo Scientific Varioskan Flash Type 3001 Microplate Reader 5250040 MFD 2012 Untested due to lack of software. Powers on confirmed and the plate tray opens/closes via the front panel controls. No further functional testing carried out. Included in the sale: Thermo Scientific Varioskan Flash microplate reader (Type 3001, REF 5250040)

The Thermo Scientific Varioskan Flash (REF 5250040) is a spectral scanning multimode microplate reader designed for laboratory research use. The REF 5250040 variant includes both top and bottom reading capability. It measures fluorescence intensity (FI), time-resolved fluorescence (TRF), absorbance, and luminescence in end-point, kinetic, and spectral measurement modes across the UV/Vis/NIR range. It is certified DLReady™ by Promega and LanthaScreen™ by Invitrogen. Ideal applications include binding assays, ADMETox, molecular biology assays, enzyme kinetic studies, ion-channel and cell signalling assays, drug discovery, genomics, proteomics, and ELISA.

Fluorescence Intensity / Time-Resolved Fluorescence:
Sample format: 6 – 1536-well plates
Wavelength selection: Double excitation and double emission monochromators
Excitation wavelength range: 200 – 1000 nm
Emission wavelength range: 270 – 840 nm
Excitation/emission bandwidth: 5 nm / 12 nm
Light source: Xenon flash lamp
Sensitivity/dynamic range (FI top reading): 6 decades, 384-well plate
Sensitivity/dynamic range (FI bottom reading): 5.5 decades, 384-well plate
Sensitivity/dynamic range (TRF top reading): 6 decades, 384-well plate

Photometry:
Sample format: 6 – 384-well plates
Wavelength selection: Double monochromators
Wavelength range: 200 – 1000 nm
Bandwidth: 5 nm
Light source: Xenon flash lamp
Linear measurement range: 0 – 4 Abs (96-well plate) at 450 nm
Accuracy: ±1% or 0.003 Abs (whichever is greater) at 400–1000 nm (0–3 Abs)
Precision: SD <0.001 Abs or CV <0.5% at 450 nm (0–3 Abs)
Read speed: 96-well plate in 15 s, 384-well plate in 45 s, 1536-well plate in 135 s

Luminometry:
Sample format: 6 – 1536-well plates (spectral scanning); 6 – 384-well plates
Wavelength range: 360 – 670 nm (spectral scanning); 270 – 840 nm
Sensitivity/dynamic range: 7 decades, flash ATP reaction, 384-well plate

Dispenser:
Number of dispensers: Up to 3, with automatic dispensing position control
Sample format: 6 – 384-well plates
Syringe size: 1 mL (standard), 5 mL (on request)
Dispensing volume: 1 – 10,000 µL in 1 µL increments (1 mL syringe)
Dispensing accuracy: <0.2 µL or 2%, whichever is greater (5–10,000 µL)

General:
Power supply: 100–240 VAC, 50/60 Hz, 200 VA
Manufactured in: Finland
Software: Thermo Scientific SkanIt Software for Varioskan Flash
Interface: RS-232 Serial / USB

Thermo Scientific Varioskan Flash User Manual Rev. 1.2 — full instrument operating and installation guide (PDF): Download PDF

Thermo Scientific Varioskan Flash product brochure — key features, specifications and ordering information (PDF): Download PDF