Condition: Used

In excellent cosmetic condition, all tests pass. Supplied with PR2 and waste bottles, Flow Test cell (non-beaded) wash tray, power cord. MFD 2017
Running windows 10, PC software MiSeq Control Software (MCS) can be downloaded at illumina's website

Speed and simplicity for targeted resequencing and small-genome sequencing

Exceptional data quality demonstrated through peerreviewed, scientific comparison • Highly automated system with simple, intuitive instrument workflow • Rapid sequencing and variant detection for time-critical studies • Adjustable read length and flow cell options provide flexibility across a broad range of applications

Introduction

The MiSeq System offers the first DNA-to-data sequencing platform integrating cluster generation, amplification, sequencing, and data analysis into a single instrument. Its small footprint—approximately two square feet—fits easily into virtually any laboratory environment (Figure 1). The MiSeq System leverages Illumina sequencing by synthesis (SBS) chemistry, a proven next-generation sequencing (NGS) technology cited in over 300,000 peer-reviewed publications—5× more than all other NGS technologies combined.1 With the power of NGS delivered in a compact footprint, the MiSeq System is the ideal platform for rapid and cost-effective genetic analysis.

Simple, intuitive NGS workflow

The MiSeq System offers straightforward, easy-to-follow instrument control software. Perform instrument operations with an intuitive touch screen interface, use plugand-play reagent cartridges with radio frequency identifier (RFID) tracking, consult on-screen video tutorials, and enjoy step-by-step guidance throughout each sequencing workflow. All MiSeq Systems include onboard data analysis and access to BaseSpace™ Sequence Hub, the Illumina genomic cloud-computing platform. BaseSpace Sequence Hub provides real-time data uploading, simple data analysis tools, internet-based run monitoring, and a secure, scalable storage solution. A suite of data analysis tools and a growing list of third-party analysis apps empower researchers to perform their own informatics. BaseSpace Sequence Hub also enables fast and easy data sharing with colleagues or customers.

Fast turnaround time

For results in hours rather than days, the combination of rapid library preparation and the MiSeq System delivers a simple, accelerated turnaround time (Figure 2). Prepare your sequencing library in as few as three hours with Illumina DNA Prep library prep reagents, then move to automated clonal amplification, sequencing, and qualityscored base calling in as few as 5.5 hours on the MiSeq System (Table 1). Sequence alignment can be completed directly on the onboard instrument computer with MiSeq Local Run Manager software or through BaseSpace Sequence Hub within three hours.

Exceptional data quality

Illumina SBS chemistry achieves exceptional data quality. This proprietary reversible terminator–based method enables the massively parallel sequencing of billions of DNA fragments, detecting single bases as they are incorporated into growing DNA strands. Fluorescent terminator dyes are imaged as each dNTP is added and then cleaved to allow incorporation of the next base. With all four reversible terminator–bound dNTPs present during each cycle, natural competition minimizes incorporation bias. Base calls are made directly from signal intensity measurements during each cycle, greatly reducing raw error rates compared to other technologies. The result is highly accurate base-by-base sequencing that virtually eliminates sequence context–specific errors, even within repetitive sequence regions or homopolymers (Figure 3).2 

Extensive suite of applications

Explore an ever increasing range of sequencing applications. With faster turnaround time and simplified workflows, the MiSeq System offers a cost-effective alternative to sequencing by capillary electrophoresis and qPCR for applications such as targeted resequencing, clone checking, amplicon sequencing, and RNA expression. Local Run Manager Software and BaseSpace Sequence Hub offer optimized analysis workflows for small genome sequencing, 16S metagenomics, RNA sequencing, targeted resequencing, and preimplantation genetic screening (PGS), as well as highly multiplexed applications such as amplicon sequencing with AmpliSeq™ for Illumina panels. Adjustable read lengths, flow cell options, and choice of single or paired-end reads allow flexibility for matching data output to a broad range of experimental needs.

Datasheet

Shipping & Returns Policy

Shipping Policy

• Processing Time: Orders are typically processed and dispatched within 1 business day.
• Shipping Methods: We offer Expedited Shipping to all destinations using UPS/DHL/Fedex/Royal Mail and other reputable carriers.
• Shipping Times: Mainland UK: 1-2 Business Days / Mainland Europe and USA: 2-3 Business Days / Rest of the World 5-7 Business Days.
• Shipping Costs: These are calculated at checkout based on the shipping method, destination and package dimensions.
• CUSTOMS AND TAXES: It is YOUR responsibility to liaise with customs in your country and ensure your package is cleared and any taxes are paid.
• Shipping Destinations: We ship worldwide.
• Tracking: Once your order is shipped, we will provide you with a tracking number via email.
• Insurance: All shipments are insured. In case of damage, please contact us via email within 48 hours of receiving the product with photographic evidence, please also ensure a report is logged with the delivery driver.

Returns Policy

• Return Period: In the event of an item being received which is 'not as specified', you can return your purchase within 14 days of delivery.
• Condition of Items: Items must be returned unused and in their original packaging.
• Return Method: All returns must be notified in writing to hello@ttinstruments.com, the email should accompany the order number, date and reason for return and must be within 14 days of delivery.
• Restocking Fees: A restocking fee of up-to 20% is applicable for items returned for any reason other than 'not as specified'.
• Refund Method: Refunds are processed to the original method of payment within 5 business days of receiving the return.
• Exchanges: We do not offer exchanges. It will either be a replacement or a refund.
• Return Shipping Costs: Return shipping costs will be borne by the customer unless the item is 'not as specified', in this case we will arrange for the return by way of a prepaid shipping label.